A complimentary approach to transcriptional investigation of cell complexity is through the regulome. The unique chemistry of ATAC-seq to measure accessible chromatin sites can be applied to single cells. These experiments identify expressed genes as well as regulatory elements. Barcoding of nuclei suspensions is achieved through the Chromium Controller with Next GEM technology. Libraries are manually prepared. Automated library preparation with the Connect system will be available in the near future.
For every project, the raw fastq files will be made available. Through our analysis pipeline, we will also provide binarized and non-binarized peak-by-cell count matrix. Associated QC metrics are included. Our expert team will review and summarize the data with the investigation team. Through collaboration we can also curate customized data analysis strategies.